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tobacco etch virus tev protease  (Addgene inc)


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    Addgene inc tobacco etch virus tev protease
    Tobacco Etch Virus Tev Protease, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk1043/pmc10614602__pnas__2307203120__sapp-6-19-24?v=Addgene+inc
    Average 92 stars, based on 13 article reviews
    tobacco etch virus tev protease - by Bioz Stars, 2026-07
    92/100 stars

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    Fig. 1. The Synthetic Processing (SynPro) system shows that prodomain cleavage can be non-cell-autonomous. (A) The SynPro system comprises an orthogonal secreted <t>protease</t> derived from <t>tobacco</t> <t>etch</t> <t>virus</t> (secTEVp) and a cognate sequence that replaces the endogenous cleavage site of Vg1 (SynPro Vg1, RSRRKR → ENLYFQS). (B) (i) Rescue percentage after 30 hpf of Mvg1 embryos injected with 50 pg of vg1, SynPro vg1, SynPro vg1 and secTEVp, or SynPro vg1 and secTEVp-KDEL mRNAs. (ii) Representative images of 30 hpf Mvg1 embryos for the indicated injection condition. Minor brain and tail defects are noted in embryos transiently rescued with mRNAs of the SynPro system. (Scale bar, 0.5 mm.) (iii) Fluorescence images of Mvg1 embryos at 50 to 60% epiboly that were injected with mRNAs for sfGFP-tagged wild-type Vg1 and secTEVp-sfCherry (Top) or secTEVp-sfCherry-KDEL (Bottom). (Scale bar, 20 μm.) (C) Schematic of transplantation assay. Mvg1 embryos were injected with 50 pg mRNA each of: (i) DONOR: cyc, SynPro vg1, and secTEVp; HOST: none; (ii) DONOR: cyc and SynPro vg1; HOST: secTEVp; (iii) DONOR: cyc and SynPro vg1; HOST: none; (iv) DONOR: none; HOST: secTEVp. All Mvg1 donor embryos were marked by also injecting 50 pg sfGFP mRNA. At high stage, before the onset of Nodal signaling, sfGFP-marked DONOR cells were transplanted to the animal pole of HOST Mvg1 embryos. (D) At 50 to 60% epiboly, chimeric embryos were fixed and immunostained for sfGFP and pSmad2. DAPI, nuclei. (Scale bar, 20 μm.)
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    Fig. 1. The Synthetic Processing (SynPro) system shows that prodomain cleavage can be non-cell-autonomous. (A) The SynPro system comprises an orthogonal secreted <t>protease</t> derived from <t>tobacco</t> <t>etch</t> <t>virus</t> (secTEVp) and a cognate sequence that replaces the endogenous cleavage site of Vg1 (SynPro Vg1, RSRRKR → ENLYFQS). (B) (i) Rescue percentage after 30 hpf of Mvg1 embryos injected with 50 pg of vg1, SynPro vg1, SynPro vg1 and secTEVp, or SynPro vg1 and secTEVp-KDEL mRNAs. (ii) Representative images of 30 hpf Mvg1 embryos for the indicated injection condition. Minor brain and tail defects are noted in embryos transiently rescued with mRNAs of the SynPro system. (Scale bar, 0.5 mm.) (iii) Fluorescence images of Mvg1 embryos at 50 to 60% epiboly that were injected with mRNAs for sfGFP-tagged wild-type Vg1 and secTEVp-sfCherry (Top) or secTEVp-sfCherry-KDEL (Bottom). (Scale bar, 20 μm.) (C) Schematic of transplantation assay. Mvg1 embryos were injected with 50 pg mRNA each of: (i) DONOR: cyc, SynPro vg1, and secTEVp; HOST: none; (ii) DONOR: cyc and SynPro vg1; HOST: secTEVp; (iii) DONOR: cyc and SynPro vg1; HOST: none; (iv) DONOR: none; HOST: secTEVp. All Mvg1 donor embryos were marked by also injecting 50 pg sfGFP mRNA. At high stage, before the onset of Nodal signaling, sfGFP-marked DONOR cells were transplanted to the animal pole of HOST Mvg1 embryos. (D) At 50 to 60% epiboly, chimeric embryos were fixed and immunostained for sfGFP and pSmad2. DAPI, nuclei. (Scale bar, 20 μm.)
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    Fig. 1. The Synthetic Processing (SynPro) system shows that prodomain cleavage can be non-cell-autonomous. (A) The SynPro system comprises an orthogonal secreted <t>protease</t> derived from <t>tobacco</t> <t>etch</t> <t>virus</t> (secTEVp) and a cognate sequence that replaces the endogenous cleavage site of Vg1 (SynPro Vg1, RSRRKR → ENLYFQS). (B) (i) Rescue percentage after 30 hpf of Mvg1 embryos injected with 50 pg of vg1, SynPro vg1, SynPro vg1 and secTEVp, or SynPro vg1 and secTEVp-KDEL mRNAs. (ii) Representative images of 30 hpf Mvg1 embryos for the indicated injection condition. Minor brain and tail defects are noted in embryos transiently rescued with mRNAs of the SynPro system. (Scale bar, 0.5 mm.) (iii) Fluorescence images of Mvg1 embryos at 50 to 60% epiboly that were injected with mRNAs for sfGFP-tagged wild-type Vg1 and secTEVp-sfCherry (Top) or secTEVp-sfCherry-KDEL (Bottom). (Scale bar, 20 μm.) (C) Schematic of transplantation assay. Mvg1 embryos were injected with 50 pg mRNA each of: (i) DONOR: cyc, SynPro vg1, and secTEVp; HOST: none; (ii) DONOR: cyc and SynPro vg1; HOST: secTEVp; (iii) DONOR: cyc and SynPro vg1; HOST: none; (iv) DONOR: none; HOST: secTEVp. All Mvg1 donor embryos were marked by also injecting 50 pg sfGFP mRNA. At high stage, before the onset of Nodal signaling, sfGFP-marked DONOR cells were transplanted to the animal pole of HOST Mvg1 embryos. (D) At 50 to 60% epiboly, chimeric embryos were fixed and immunostained for sfGFP and pSmad2. DAPI, nuclei. (Scale bar, 20 μm.)
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    Fig. 1. The Synthetic Processing (SynPro) system shows that prodomain cleavage can be non-cell-autonomous. (A) The SynPro system comprises an orthogonal secreted <t>protease</t> derived from <t>tobacco</t> <t>etch</t> <t>virus</t> (secTEVp) and a cognate sequence that replaces the endogenous cleavage site of Vg1 (SynPro Vg1, RSRRKR → ENLYFQS). (B) (i) Rescue percentage after 30 hpf of Mvg1 embryos injected with 50 pg of vg1, SynPro vg1, SynPro vg1 and secTEVp, or SynPro vg1 and secTEVp-KDEL mRNAs. (ii) Representative images of 30 hpf Mvg1 embryos for the indicated injection condition. Minor brain and tail defects are noted in embryos transiently rescued with mRNAs of the SynPro system. (Scale bar, 0.5 mm.) (iii) Fluorescence images of Mvg1 embryos at 50 to 60% epiboly that were injected with mRNAs for sfGFP-tagged wild-type Vg1 and secTEVp-sfCherry (Top) or secTEVp-sfCherry-KDEL (Bottom). (Scale bar, 20 μm.) (C) Schematic of transplantation assay. Mvg1 embryos were injected with 50 pg mRNA each of: (i) DONOR: cyc, SynPro vg1, and secTEVp; HOST: none; (ii) DONOR: cyc and SynPro vg1; HOST: secTEVp; (iii) DONOR: cyc and SynPro vg1; HOST: none; (iv) DONOR: none; HOST: secTEVp. All Mvg1 donor embryos were marked by also injecting 50 pg sfGFP mRNA. At high stage, before the onset of Nodal signaling, sfGFP-marked DONOR cells were transplanted to the animal pole of HOST Mvg1 embryos. (D) At 50 to 60% epiboly, chimeric embryos were fixed and immunostained for sfGFP and pSmad2. DAPI, nuclei. (Scale bar, 20 μm.)
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    Image Search Results


    Fig. 1. The Synthetic Processing (SynPro) system shows that prodomain cleavage can be non-cell-autonomous. (A) The SynPro system comprises an orthogonal secreted protease derived from tobacco etch virus (secTEVp) and a cognate sequence that replaces the endogenous cleavage site of Vg1 (SynPro Vg1, RSRRKR → ENLYFQS). (B) (i) Rescue percentage after 30 hpf of Mvg1 embryos injected with 50 pg of vg1, SynPro vg1, SynPro vg1 and secTEVp, or SynPro vg1 and secTEVp-KDEL mRNAs. (ii) Representative images of 30 hpf Mvg1 embryos for the indicated injection condition. Minor brain and tail defects are noted in embryos transiently rescued with mRNAs of the SynPro system. (Scale bar, 0.5 mm.) (iii) Fluorescence images of Mvg1 embryos at 50 to 60% epiboly that were injected with mRNAs for sfGFP-tagged wild-type Vg1 and secTEVp-sfCherry (Top) or secTEVp-sfCherry-KDEL (Bottom). (Scale bar, 20 μm.) (C) Schematic of transplantation assay. Mvg1 embryos were injected with 50 pg mRNA each of: (i) DONOR: cyc, SynPro vg1, and secTEVp; HOST: none; (ii) DONOR: cyc and SynPro vg1; HOST: secTEVp; (iii) DONOR: cyc and SynPro vg1; HOST: none; (iv) DONOR: none; HOST: secTEVp. All Mvg1 donor embryos were marked by also injecting 50 pg sfGFP mRNA. At high stage, before the onset of Nodal signaling, sfGFP-marked DONOR cells were transplanted to the animal pole of HOST Mvg1 embryos. (D) At 50 to 60% epiboly, chimeric embryos were fixed and immunostained for sfGFP and pSmad2. DAPI, nuclei. (Scale bar, 20 μm.)

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Molecular mechanisms controlling the biogenesis of the TGF-β signal Vg1.

    doi: 10.1073/pnas.2307203120

    Figure Lengend Snippet: Fig. 1. The Synthetic Processing (SynPro) system shows that prodomain cleavage can be non-cell-autonomous. (A) The SynPro system comprises an orthogonal secreted protease derived from tobacco etch virus (secTEVp) and a cognate sequence that replaces the endogenous cleavage site of Vg1 (SynPro Vg1, RSRRKR → ENLYFQS). (B) (i) Rescue percentage after 30 hpf of Mvg1 embryos injected with 50 pg of vg1, SynPro vg1, SynPro vg1 and secTEVp, or SynPro vg1 and secTEVp-KDEL mRNAs. (ii) Representative images of 30 hpf Mvg1 embryos for the indicated injection condition. Minor brain and tail defects are noted in embryos transiently rescued with mRNAs of the SynPro system. (Scale bar, 0.5 mm.) (iii) Fluorescence images of Mvg1 embryos at 50 to 60% epiboly that were injected with mRNAs for sfGFP-tagged wild-type Vg1 and secTEVp-sfCherry (Top) or secTEVp-sfCherry-KDEL (Bottom). (Scale bar, 20 μm.) (C) Schematic of transplantation assay. Mvg1 embryos were injected with 50 pg mRNA each of: (i) DONOR: cyc, SynPro vg1, and secTEVp; HOST: none; (ii) DONOR: cyc and SynPro vg1; HOST: secTEVp; (iii) DONOR: cyc and SynPro vg1; HOST: none; (iv) DONOR: none; HOST: secTEVp. All Mvg1 donor embryos were marked by also injecting 50 pg sfGFP mRNA. At high stage, before the onset of Nodal signaling, sfGFP-marked DONOR cells were transplanted to the animal pole of HOST Mvg1 embryos. (D) At 50 to 60% epiboly, chimeric embryos were fixed and immunostained for sfGFP and pSmad2. DAPI, nuclei. (Scale bar, 20 μm.)

    Article Snippet: Commercially available sequences for tobacco etch virus protease (TEVp, Addgene Plasmid #8835), tobacco vein mottling virus protease (TVMVp; Addgene Plasmid #8832), and human rhinovirus 3C protease (HRV 3Cp; Addgene Plasmid #78571) were gifts from David Waugh (National Cancer Institute, Frederick, MD) (71–73).

    Techniques: Derivative Assay, Virus, Sequencing, Injection, Fluorescence, Transplantation Assay